(Lightning storms) Crazy Weight Loss Diet Fads
No commentsBy Paul Strachocki
You may see the hottest models in the world supporting the great new diet fad. You may even see a famous fat person turned skinny talking about how the diet fad made it possible. Some of these celebrities may even have diet fads marketed under their names. There is a strong pull inside of us to believe them. After all, they are society’s ideal for what we should look like. But what exactly do they know about nutrition and health? How many years have they remained skinny? As a society, we really need to learn how to analyze celebrity endorsements more critically.
Many of the more recent diet crazes have been driven by book sales. To fight back, nutrition experts such as Jo-Ann Heslin and Annette B. Natow have written books of their own urging more caution in the acceptance of fad diets. These ‘miracle’ weight loss diets often throw out a lot of information that is assumed to be scientific, but in reality is at best speculative information. Many times these diets gain quick acclaim and everybody reads fad diet books instead of legitimate books written by nutritionists and health scientists.
While celebrities are typically the faces of these fad diets, there is usually someone else behind them. When these people can’t find celebrity endorsers, they will often try to market their fad diet books themselves. For example, you may have heard of the grapefruit diet, cabbage soup diet, and eggs and fasting diet. The lack of scientific data behind such extreme fad diets makes them dangerous.
The cabbage soup diet is an interesting example. It has yet to be endorsed by any medical establishment. In fact, the American Heart Association says it can be harmful. The people who created the diet say they have witnessed 15lbs of weight loss in a week, without telling that it was likely mainly water weight that was lost. Once you return to drinking and eating the way you did before, your weight will likely go up higher than ever. Worse still, you may suffer from stomach cramps and diarrhea while you’re on the diet.
You’ll find a variety of other really strange fad diets out their too. One set of fad diets is based on an idea that the types of foods you mix during your meals determines whether you gain or lose weight. Another set of fad diets goes into mind over matter types of ideas. One product even claims to make you dream more so you’ll lose more weight while you sleep.
A really common fad diet ploy is to sell you a gimmick food that is supposed to get rid of your hunger when eaten in place of a meal. You’ve probably seen such weight loss candy bars, teas, and milkshakes in your local grocery store. How many skinny people do you know that are taking part in these types of fad diets? There is no scientific evidence from reputable sources to support these claims. The reality is that they’re the works of cunning individuals who know how profitable your desperation can be. Just remember that such fad diets are no substitute for patience and self-discipline.
After years of neglect, I found that my health had suffered. My website,
Paul Strachocki
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Fragment specificity and antibody selection
By Brigette Federico
Immunoassays such as Western blotting and ELISA often rely on a two-stage technique. A primary antibody (1o Ab) is used to bind directly to the protein of interest. A secondary antibody (2o Ab), labelled with a detection tag such as a fluorescent dye or colourmetric enzyme, is then applied. This binds to a site on the primary Ab, allowing indirect detection of the antigen.
Single stage assays have been developed, in which primary immunoglobulins are tagged, allowing both probing and detection to take place from the same molecule. However, the indirect approach is still preferred, for many reasons. One-step 1o Abs are custom-built and thus expensive. In addition, more than one 2o Ab can target the primary protein producing clearer results.
Primary Abs are typically purchased from antibody suppliers in monoclonal form. These are derived from a single cell-line and are totally homogenous. By contrast, most secondary proteins are polyclonal. Here, animals are injected with a specific peptide antigen of a particular species, a secondary immune response is triggered and in this way a whole plethora of proteins are produced unique for that antigen.
The difference between monoclonal and polyclonal proteins is that, while a monoclonal only recognises one specific epitope, polyclonals can recognise several. The primaries are raised against a specific antigen, and are unlabelled. 2o Abs are raised against primaries, and are labelled, or conjugated, with a reporter enzyme or one of the new fluorescent products such as Dylight.
Sometimes best results are obtained by using antibody fragments, such as an Fab fragment or gamma light chain, as the primary Ab rather than the entire protein. This can improve signal-to-noise ratio in assays, and improve binding to the antigen. The 2o Ab will be specific to Fab.
The secondary antibodies supplied by us at Novus Biologicals are available for a wide range of specie and primary proteins.
The Article is written by novusbio.com providing antibody database and antibodies Services. Visit http://www.novusbio.com for more information on novusbio.com Products & Services___________________________Copyright information
This article is free for reproduction but must be reproduced in its entirety, including live links & this copyright statement must be included. Visit novusbio.com for more services!
Thursday, January 28th, 2010 at 3:50 am and is filed under science. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.
